By Walter Maechtle
This booklet is split into chapters overlaying instrumentation, sedimentation pace runs, density gradient runs, program examples and destiny advancements. specifically, the specific software bankruptcy demonstrates the flexibility and gear of AUC through many fascinating and critical commercial examples. therefore the ebook concentrates on sensible points instead of information of centrifugation thought.
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Extra info for Analytical Ultracentrifugation of Polymers and Nanoparticles (Springer Laboratory)
2 Analytical Ultracentrifugation, Instrumentation Centrifugation is a common technical process for the separation of materials consisting of two (or more) compounds having different sizes and/or showing different densities, such as dissolved macromolecules in a solvent or dispersed particles in a liquid. A centrifuge is basically an apparatus to create centrifugal ﬁelds by fast rotation of a rotor. The special design of centrifuges allows us letting these ﬁelds act on samples inside the rotor, which leads to a sedimentation (or ﬂotation) of the dissolved/dispersed macromolecules/particles and also to their fractionation, if the macromolecules/particles are different in size and/or density.
Fig. 12. 4 Detectors 41 Turbidity Detectors Similarly to the case of the ﬂuorescence detector, to our knowledge there are just two types of turbidity (= light scattering) detectors [13, 14]. Both are user-made, and therefore not commercially available. However, it is not very difﬁcult to build such a turbidity detector. Again, only a short description is given here. The principle of a turbidity measurement is comparable to that of an absorption detector, but it is much simpler, because the absorption A = lg(I0 /I) is measured at only one ﬁxed radial position rslit .
13 shows a schematic diagram of the turbidity detector developed at BASF, which is part of a particle sizer. The turbid dispersion to be analyzed is diluted to about c = 1g/l, and placed into the centerpiece of the 3-mm mono-sector cell. 2mm wide slit arranged perpendicularly to the radius of the rotor. The slit picks out a beam from the entering parallel monochromatic light. This is simply created by using a stabilized incandescent lamp as a light source, and a monochromatic light ﬁlter (λ = 546nm) after the condensing lens.